Clotilde Costa Nogueira

Head of the Liquid Biopsy Line of the Roche-Chus Joint Unit

Dr.Clotilde Costa obtained her doctorate in 2011 at the Department of Molecular Oncology CIEMAT – Autonomous University of Madrid. Her thesis focused on the study of genes involved in cell cycle – Retinoblastoma Family and E2F factors – and their role in squamous cell carcinoma in transgenic animals. In 2013 she joined the Translational Medical Oncology Group in the Health Research Institute of Santiago de Compostela in the European InveNNta project, focused on the isolation and characterisation of circulating tumour cells in metastatic patients.

Currently, she lead the Liquid Biopsy Line of the Roche-Chus Joint Unit.

Experience: animal models, cell cultures, primary cultures, molecular and cell biology.

Interests: CTCs, metastasis, oncology, biomarkers, molecular biology.


Phone: +34 981 95 56 11


Costa C, Muinelo-Romay L, Cebey-López V, Pereira-Veiga T, Martínez-Pena I, Abreu M, Abalo A, Lago-Lestón RM, Abuín C, Palacios P, Cueva J, Piñeiro R, López-López R. Analysis of a Real-World Cohort of Metastatic Breast Cancer Patients Shows Circulating Tumor Cell Clusters (CTC-clusters) as Predictors of Patient Outcomes. Cancers (Basel). 2020 Apr 29;12(5). pii: E1111. doi: 10.3390/cancers12051111. PubMed PMID: 32365530.

Costa C, Dávila-Ibáñez AB. Methodology for the Isolation and Analysis of CTCs. Adv Exp Med Biol. 2020;1220:45-59. doi: 10.1007/978-3-030-35805-1_4. Review. PubMed PMID: 32304079

Carmona-Ule, C. Abuín-Redondo, C. Costa, R. Piñeiro, T. Pereira-Veiga, I. Martínez-Pena, P. Hurtado, R. López-López, M. de la Fuente, A.B. Dávila-Ibáñez. Nanoemulsions to support ex vivo cell culture of breast cancer circulating tumor cells. Materials Today Chemistry, vol 16, 2020,

Abreu M, Cabezas-Sainz P, Pereira-Veiga T, Falo C, Abalo A, Morilla I, Curiel T, Cueva J, Rodríguez C, Varela-Pose V, Lago-Lestón R, Mondelo P, Palacios P, Moreno-Bueno G, Cano A, García-Caballero T, Pujana MÁ, Sánchez-Piñón L, Costa C, López R, Muinelo-Romay L. Looking for a Better Characterization of Triple-Negative Breast Cancer by Means of Circulating Tumor Cells. J Clin Med. 2020 Jan 27;9(2). pii: E353. doi: 10.3390/jcm9020353. PubMed PMID: 32012729; PubMed Central PMCID: PMC7074553.

Pereira-Veiga T, Martínez-Fernández M, Abuin C, Piñeiro R, Cebey V, Cueva J, Palacios P, Blanco C, Muinelo-Romay L, Abalo A, Costa C, López-López R. CTCs Expression Profiling for Advanced Breast Cancer Monitoring. Cancers (Basel). 2019 Dec 4;11(12). pii: E1941. doi: 10.3390/cancers11121941. PubMed PMID: 31817194; PubMed Central PMCID: PMC6966538.

Ribeiro-Samy S, Oliveira MI, Pereira-Veiga T, Muinelo-Romay L, Carvalho S, Gaspar J, Freitas PP, López-López R, Costa C, Diéguez L. Fast and efficient microfluidic cell filter for isolation of circulating tumor cells from unprocessed whole blood of colorectal cancer patients. Sci Rep. 2019 May 29;9(1):8032. doi: 10.1038/s41598-019-44401-1. PubMed PMID: 31142796; PubMed Central PMCID: PMC6541613.

Pereira-Veiga T, Abreu M, Robledo D, Matias-Guiu X, Santacana M, Sánchez L, Cueva J, Palacios P, Abdulkader I, López-López R, Muinelo-Romay L, Costa C. CTCs-derived xenograft development in a triple negative breast cancer case. Int J Cancer. 2019 May 1;144(9):2254-2265. doi: 10.1002/ijc.32001. Epub 2018 Dec 16. PubMed PMID: 30450632.

EGFR-Based Immunoisolation as a Recovery Target for Low-EpCAM CTC Subpopulation.Vila A, Abal M, Muinelo-Romay L, Rodriguez-Abreu C, Rivas J, López-López R, Costa C.PLoS One. 2016 Oct 6;11(10):e0163705. doi: 10.1371/journal.pone.0163705.PMID: 27711186

M-Trap: Exosome-Based Capture of Tumor Cells as a New Technology in Peritoneal la Fuente A, Alonso-Alconada L, Costa C, Cueva J, Garcia-Caballero T, Lopez-Lopez R, Abal M.J Natl Cancer Inst. 2015 Jul 6;107(9). pii: djv184. doi: 10.1093/jnci/djv184. Print 2015 Sep.PMID: 26150590

Biosensors for the detection of circulating tumour cells.Costa C, Abal M, López-López R, Muinelo-Romay L.Sensors (Basel). 2014 Mar 10;14(3):4856-75. doi: 10.3390/s140304856. Review.PMID: 24618729

Deregulation of the pRb-E2F4 axis alters epidermal homeostasis and favors tumor development.Costa C, Santos M, Martínez-Fernández M, Lorz C, Lázaro S, Paramio JM.Oncotarget. 2016 Sep 30. doi: 10.18632/oncotarget.12362.PMID: 27708231

In vivo disruption of an Rb-E2F-Ezh2 signaling loop causes bladder cancer.Santos M, Martínez-Fernández M, Dueñas M, García-Escudero R, Alfaya B, Villacampa F, Saiz-Ladera C, Costa C, Oteo M, Duarte J, Martínez V, Gómez-Rodriguez MJ, Martín ML, Fernández M, Viatour P, Morcillo MA, Sage J, Castellano D, Rodriguez-Peralto JL, de la Rosa F, Paramio JM.Cancer Res. 2014 Nov 15;74(22):6565-77. doi: 10.1158/0008-5472.CAN-14-1218. Epub 2014 Sep 24.PMID: 25252918

Skin Tumors Rb(eing) Uncovered.Costa C, Paramio JM, Santos M.Front Oncol. 2013 Dec 17;3:307. doi: 10.3389/fonc.2013.00307. Review.PMID: 24381932

The Rho exchange factors Vav2 and Vav3 favor skin tumor initiation and promotion by engaging extracellular signaling loops.Menacho-Márquez M, García-Escudero R, Ojeda V, Abad A, Delgado P, Costa C, Ruiz S, Alarcón B, Paramio JM, Bustelo XR.PLoS Biol. 2013 Jul;11(7):e1001615. doi: 10.1371/journal.pbio.1001615. Epub 2013 Jul 23.PMID: 23935450

A novel tumor suppressor network in squamous malignancies.Costa C, Santos M, Segrelles C, Dueñas M, Lara MF, Agirre X, Prosper F, García-Escudero R, Paramio JM.Sci Rep. 2012;2:828. doi: 10.1038/srep00828. Epub 2012 Nov 9.PMID: 23145321

E2F1 loss induces spontaneous tumour development in Rb-deficient epidermis.Costa C, Santos M, Martínez-Fernández M, Dueñas M, Lorz C, García-Escudero R, Paramio JM.Oncogene. 2013 Jun 13;32(24):2937-51. doi: 10.1038/onc.2012.316. Epub 2012 Aug 13.PMID: 22890321

EMT and induction of miR-21 mediate metastasis development in Trp53-deficient tumours.Bornachea O, Santos M, Martínez-Cruz AB, García-Escudero R, Dueñas M, Costa C, Segrelles C, Lorz C, Buitrago A, Saiz-Ladera C, Agirre X, Grande T, Paradela B, Maraver A, Ariza JM, Prosper F, Serrano M, Sánchez-Céspedes M, Paramio JM.Sci Rep. 2012;2:434. doi: 10.1038/srep00434. Epub 2012 May 31.PMID: 22666537

The thyroid hormone receptors as modulators of skin proliferation and inflammation.Contreras-Jurado C, García-Serrano L, Gómez-Ferrería M, Costa C, Paramio JM, Aranda A.J Biol Chem. 2011 Jul 8;286(27):24079-88. doi: 10.1074/jbc.M111.218487. Epub 2011 May 12.PMID: 21566120

 A functional role of RB-dependent pathway in the control of quiescence in adult epidermal stem cells revealed by genomic profiling.Lorz C, García-Escudero R, Segrelles C, Garín MI, Ariza JM, Santos M, Ruiz S, Lara MF, Martínez-Cruz AB, Costa C, Buitrago-Pérez A, Saiz-Ladera C, Dueñas M, Paramio JM.Stem Cell Rev. 2010 Jun;6(2):162-77. doi: 10.1007/s12015-010-9139-0.PMID: 20376578

 In vivo transplantation of genetically modified mouse embryonic epidermis.Martínez-Cruz AB, Costa C, Saiz C, Paramio JM, Santos M.Methods Mol Biol. 2010;585:361-7. doi: 10.1007/978-1-60761-380-0_25.PMID: 19908016

 Transgenic mice expressing constitutively active Akt in oral epithelium validate KLFA as a potential biomarker of head and neck squamous cell carcinoma.Moral M, Segrelles C, Martínez-Cruz AB, Lorz C, Santos M, García-Escudero R, Lu J, Buitrago A, Costa C, Saiz C, Ariza JM, Dueñas M, Rodriguez-Peralto JL, Martinez-Tello FJ, Rodriguez-Pinilla M, Sanchez-Cespedes M, Digiovanni J, Paramio JM.In Vivo. 2009 Sep-Oct;23(5):653-60.PMID: 19779097

Spontaneous tumor formation in Trp53-deficient epidermis mediated by chromosomal instability and inflammation.Martínez-Cruz AB, Santos M, García-Escudero R, Moral M, Segrelles C, Lorz C, Saiz C, Buitrago-Pérez A, Costa C, Paramio JM.Anticancer Res. 2009 Aug;29(8):3035-42.PMID: 19661312

Akt activation synergizes with Trp53 loss in oral epithelium to produce a novel mouse model for head and neck squamous cell carcinoma.Moral M, Segrelles C, Lara MF, Martínez-Cruz AB, Lorz C, Santos M, García-Escudero R, Lu J, Kiguchi K, Buitrago A, Costa C, Saiz C, Rodriguez-Peralto JL, Martinez-Tello FJ, Rodriguez-Pinilla M, Sanchez-Cespedes M, Garín M, Grande T, Bravo A, DiGiovanni J, Paramio JM.Cancer Res. 2009 Feb 1;69(3):1099-108. doi: 10.1158/0008-5472.CAN-08-3240. Epub 2009 Jan 27.PMID: 19176372

Synthesis and expression of CDw75 antigen in human colorectal cancer.Costa-Nogueira C, Villar-Portela S, Cuevas E, Gil-Martín E, Fernández-Briera A.BMC Cancer. 2009 Dec 10;9:431. doi: 10.1186/1471-2407-9-431.PMID: 20003255

Gene expression profiling of mouse p53-deficient epidermal carcinoma defines molecular determinants of human cancer malignancy.García-Escudero R, Martínez-Cruz AB, Santos M, Lorz C, Segrelles C, Garaulet G, Saiz-Ladera C, Costa C, Buitrago-Pérez A, Dueñas M, Paramio JM.Mol Cancer. 2010 Jul 14;9:193. doi: 10.1186/1476-4598-9-193.PMID: 20630075